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KMID : 0881720190340050413
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Journal of Food Hygiene and Safety
2019 Volume.34 No. 5 p.413 ~ p.420
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Method Development for Determination of Chlorogenic Acid and Arbutin Contents in Fruits by UHPLC-MS/MS
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Choi Young-Ju
Jeon Jong-Sup
Kim Woon-Ho
Jung You-Jung
Ryu Ji-Eun
Choi Jong-Chul
Chae Kyung-Suk
Lee Jin-Hee
Do Young-Sook
Park Yong-Bae
Yoon Mi-Hye
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Abstract
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In this study, a sample preparation method and a simultaneous determination method by ultra-high performance liquid chromatography coupled with tandem mass spectrometry for 9 isomers of chlorogenic acid and arbutin in fruits were developed. The samples were extracted using 90% methanol (pH 3.0), with the solutions being shaken and then sonicated for 10 min each. After centrifugation at 4,000 rpm for 10 min, the extraction was concentrated under a vacuum at 40°C using a vacuum evaporator. The residue was dissolved in 5 mL of 5% methanol and filtered through a 0.45 μm membrane before UHPLC-MS/MS analysis. The separations were performed on a C18 column with gradient elution of water (containing 0.1% formic acid) and methanol (containing 0.1% formic acid). The specificity, linearity, limit of detection, limit of quantification, accuracy, and precision of the proposed methods were also evaluated.
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KEYWORD
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Chlorogenic acid, Arbutin, Fruit, UHPLC-MS/MS
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